Yes, and in my case I'm creating the rules by range, and why I need the DHCP to deliver the IP in the new range.
How can your DHCP server hand out an address to a subnet range it can't even talk too??
I am still curious as to what you would like to achieve....
Do you have some device on your network that can separate access to other workstations on the subnet based on an IP address range? (not a separate subnet) What sort of "rules" are you creating?
If you have such a device, I would like to know what it is.
If you intend to do the separation based on separate subnet. (which is not accessible from the other subnet.) You will need to do as Charlie says and physically separate them. You will then need to put a router between them that allows access only to the shared resources that they both need access too. This can be done with separate hardware and wiring (switches, etc) or with switches with Vlan tagged ports.
*A router can be used to allow 'any' two networks to talk to each other, not just from the WAN (internet) to the LAN.